The following steps are the most effective community-vetted solutions for resolving this issue on Windows. 1. The "Clean Reinstall" (Crucial Step)
In short: your DAW wants to use the Focusrite, but something else is blocking access or the connection is unstable. cannot open focusrite usb asio error code 0x54f
Go to Sound Settings > More Sound Settings > Properties > Advanced and ensure "Allow applications to take exclusive control of this device" is checked. 4. Application-Specific Notes The following steps are the most effective community-vetted
If none of these steps resolve the issue, you may want to: cannot open focusrite usb asio error code 0x54f