Shimadzu Spd20a - User Manual

The Shimadzu SPD-20A Go to product viewer dialog for this item.

1. Installation and Start-up: Carefully unpack and install the instrument, following the manufacturer's guidelines. Turn on the SPD-20A and allow it to warm up for a few minutes before use.
2. Setting Parameters: Use the intuitive menu system to set the desired wavelength, sensitivity, and response time. Refer to the user manual for detailed instructions on navigating the menu and setting parameters.
3. Sample Injection: Inject your sample into the HPLC system, ensuring that the flow rate and mobile phase composition are compatible with the SPD-20A.
4. Data Acquisition: The instrument will begin measuring absorbance values, which can be displayed on the LCD screen or output to a connected data processor or printer.

• Shimadzu Corporation. (2010). SPD-20A/20AV Prominence UV-Vis Detector Instruction Manual (Part No. 228-25000-91).
• Shimadzu Service Guide: SPD-20A Field Repair Manual (Restricted to authorized technicians).

The Bubble Fix:

If you see "sawtooth" or regular spikes in your data, it's usually air in the cell. A common lab trick is to apply light backpressure to the cell outlet using a syringe or specific tubing to force the bubbles out. SPD-20A vs. SPD-20AV If you have the shimadzu spd20a user manual