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The primary function of Sone166 lies in its contribution to the endomembrane system. In the crowded environment of a eukaryotic cell, the efficient movement of proteins and lipids between organelles is essential for survival. Sone166 acts as a scaffold or regulatory factor that ensures cargo reaches its destination—whether that be the Golgi apparatus, the vacuole, or the plasma membrane. This process, known as vesicle trafficking, is a hallmark of cellular health. When Sone166 is absent or mutated, the "postal system" of the cell breaks down, leading to the accumulation of misfolded proteins and cellular stress.

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